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mcherry sec61b  (Addgene inc)


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    Addgene inc mcherry sec61b
    Mcherry Sec61b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcherry sec61b/product/Addgene inc
    Average 93 stars, based on 82 article reviews
    mcherry sec61b - by Bioz Stars, 2026-06
    93/100 stars

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    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, <t>sec61b-mCherry,</t> and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.
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    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, <t>sec61b-mCherry,</t> and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.
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    Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, sec61b-mCherry, and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.

    Journal: Journal of Virology

    Article Title: Visualization and tracking of tubule-derived, fluorescent-labeled NS1 as a marker of bluetongue virus in living cells

    doi: 10.1128/jvi.00896-25

    Figure Lengend Snippet: Subcellular localization and aggresome formation of NS1. ( a and b ) BSR cells were transfected with the lifeact-mCherry, sec61b-mCherry, and LAMP1-mCherry expression plasmids for 12 h, infected with BTV1-NS1-552eGFP (MOI = 0.1), fixed, and then infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1) and probed with antibodies against β-tubulin and γ-tubulin to label cell microtubules and the MTOC (red). Hoechst 33342 staining (blue). Bar, 20 µm. ( c ) BSR cells were infected with BTV1-NS1-552eGFP (MOI = 0.1) or BTV1-WT (MOI = 0.1), fixed, and probed with antibodies against vimentin (red). The nuclei were stained with Hoechst 33342 (blue). Bar, 20 µm.

    Article Snippet: Lifeact-mCherry (catalog no. 193300), sec61b-mCherry (catalog no. 90994), and LAMP1-mCherry (catalog no. 45147) were purchased from Addgene (Watertown, MA, USA).

    Techniques: Transfection, Expressing, Infection, Staining